Flex Station 3
Location
B50 McCourtney
Typical Use
| Read modes | Absorbance Fluorescence (top/bottom read) Luminescence (top/bottom read) Time-Resolved Fluorescence (top/bottom read) Fluorescence Polarization (top read) HTRF (top/bottom read) |
| Wavelength ranges | Abs: 200 - 1000 nm FL: 250 - 850 nm Lumi: 250 - 850 nm TRF: 250 - 850 nm FP: 400 - 750 nm |
| Wavelength selection | Monochromator tunable in 1-nm increments |
| Absorbance photometric accuracy/linearity | < ±1.0% and ±0.006 OD (0.0-2.0 OD) |
| Absorbance photometric precision/repeatability | < ±1.0% and ±0.003 OD (0.0-2.0 OD) |
| Sensitivity top read optimized | FL: 1 fmol/well in 200 μL FITC 96 well (< 5 pM), 2 fmol/well in 100 μL FITC 384 well (< 20 pM) Lumi: < 2 fg/well for firefly luciferase in 96 and 384 well TRF: 100 fM europium in 96 and 384 well FP: < 5 mP standard deviation at 1 nM fluorescein in 96 and 384 well |
| Light source(s) | Xenon flash lamp |
| Detector(s) | Silicon photodiode Photomultiplier tube |
| Read types (endpoint, kinetic, etc) | Endpoint Kinetic Spectrum scan Well scan |
| Read times | Abs: 17 seconds FL: 15 seconds Lumi: 17 seconds TRF: 15 seconds FP: 44 seconds |
| Plate type(s) | 6-384 well plates |
| Temperature control | Ambient + 4°C to 45°C |
-from: https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/flexstation-3-reader
Internal Run Rates
- See iLab
External Run Rates
- See iLab
Instrument Availability
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